Application of staphylococcus protein A in the affinity chromatography and immunoadsorption
DOI:
https://doi.org/10.18372/2306-6407.2.5521Keywords:
affinity chromatography, Staphylococcus protein А (SPA), antibodies, immunoglobulinsAbstract
Protein A is native or recombinant proteins of microbial origin that bind to mammalian immunoglobulins. In the work characteristics of protein A, affinity sorbents on its basis, advantages of protein A for application as ligands in the affinity chromatography for antibodies purification, extraction of different antibody fractions from blood serum, antibodies and immune complexes from blood plasma of ill people in the process of immunoadsorption.References
Turkovi J. Bioaffinity chromatography / J. Turkovi – Netherlands: Elsevier Science Publishers, 1993. – 800 p.
Boi C. Performance of a new protein a affinity membrane for the primary recovery of antibodies / C. Boi, S. Dimartino, G.C. Sarti // Biotechnological Programs. – 2008. – V. 24. – № 3. – P. 640–647.
Protein A immunoadsorption in renal transplantation patients with vascular rejection / [H. Hickstein, G. Korten, R. Bast et al.] // Transfusion Sciences. – 1998. –№ 19. – P. 53–57.
Crystal structure of a Staphylococcus aureus protein. A domain complexed with the Fab fragment of a human IgM antibody: Structural basis for recognition of B-cell receptors and superantigen activity / [M. Graille, E. A. Stura, A. L. Corper et al.] // PNAS. – 2000. – P. 5399–5404.
Pat. 6,548,639 B1 USА PCT/SE98/02036. IgG binding protein from Staphylococcus and nucleotide sequence encoding this protein / Frykberg L.; Uppsala. – № 09/554,080, decl. 12.05.00, publ.15.04.03.
Nord K. Protein A chromatography for antibody purification / K. Nord, S. Hober, M. Linhult // Journal of Chromatography B. – 2007. – № 848. – P. 40–47.
Gottschalk U. Process scale purification of antibodies / U. Gottschalk – Hoboken: Wiley & Sons, 2009. – 430 p.
Experience With Protein A-Immunoadsorption in Treatment-Resistant Adult Immune Thrombocytopenic Purpura / [H.W. Snyder, S.K. Cochran, J.P. Balint et al.] // Blood. – 1992. – 79, № 9. – P. 2237–2245.
McMillan R. Chronic idiopathic thrombocytopenic purpura / R. McMillan // English Journal of Medicine. – 1981. – № 304. – p. 1135 – 1147.
Clair St. W.E. Rheumatoid Arthritis / St. W.E.Clair, D.S. Pisetsky, B.F. Haynes. – [1st ed.] – Lippincott: Williams & Wilkins, 2004. – 555 p.
Schwenger V. Immunoadsorption in nephrology and kidney transplantation / V. Schwenger, C. Morath // Nephrol Dial Transplant. – 2010. – № 25. – Р. 2407–2413.
Effects of Protein A Immunoadsorption in Patients with Chronic Dilated Cardiomyopathy / [A.O. Doesch, S. Mueller, M. Konstandin et al.] // Journal of Clinical Apheresis. – 2010. – №. 25. – Р. 315–322.
Andrew S.M. Current protocols in cell biology / S.M. Andrew, J.A. Titus. – UK: John Wiley & Sons, Inc. – 2000. – 432 p.
Josic D. Analytical and preparative methods for purification of antibodies / D. Josic, Y.-P. Lim // Food technol. biotechnol. – 2001. –V. 39. – № 3. – P. 215–226.
Antibody purification handbook. – UK: Amersham Biosciences, 2002.
Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2, AND IgG2b immunoglobulins from mouse serum using protein A-Sepharose / P. L. Ey, S. J. Prowse, C. R. Jenkin // Immunochemistry. – 1978. – V. 15. – P. 429 – 436.
