Methods for extraction and purification of phycobiliproteins

M. Y. Pylypenko, L. P. Kuzmina


Phycobiliproteins are pigments of protein nature present in cyanobacteria, red algae and cryptomonads. They are widely used in biotechnology, foods, pharmacology, cosmetics, etc. Different methods for extraction and purification of phycobiliproteins have been studied for many years, but no standard technique exists. This review highlights an overview on the basic methods used for extraction and purification of phycobiliproteins.


phycobiliproteins; extraction; purification; phycoerythrin; phycocyanin; allophycocyanin


Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent / [Manirafasha E., Ndikubwimana T., Zeng X. et al.] // Biochemical Engineering Journal. – 2016. – № 109. – P. 282–296.

Production of C-phycocyanin and its potential applications / [Kuddus M., Singh P., Thomas G., Ali A.] // Biotechnology of Bioactive Compounds: Sources and Applications. – NY: John Wiley & Sons, 2015. – P. 283–299.

Phycobilisomes linker family in cyanobacterial genomes: Divergence and evolution / [Guan X., Qin S., Zhao F. et al.] // International Journal of Biological Sciences. – 2007. – № 3. – P. 434–445.

Mogany T. Optimization of Culture Conditions and Extraction Method for Phycocyanin Production from a Hypersaline Cyanobacterium: the dissertation for the degree of Master of Technology: Biotechnology / T. Mogany. – Durban, 2014. – 179 p.

Electromagnetic biostimulation of phycobiliproteins content in cyanobacteria / [Tymoshenko L.S., Vasylchenko O.A., Lishchuk A.V. et al.] // Біологічні дослідження – 2017: Збірник наукових праць. – Житомир: ПП «Рута», 2017. – P. 351–352.

Kaur G. Algal Biology and Biotechnology / G. Kaur. – New Delhi: I. K. International Pvt Ltd, 2009 – 266 р.

C-phycocyanin extraction from Spirulina platensis wet biomass / [Moraes C. C., Sala L., Cerveira G. P., Kalil S. J.] // Brazilian Journal of Chemical Engineering. – 2011. – № 1. – P. 45–49.

Cell disruption for microalgae biorefineries / [Günerken E., D'Hondt E., Eppink M.H.M. et al.] // Biotechnology Advances. – 2015. – № 2. – P. 243–260.

Isolation, purification and characteristics of R-phycoerythrin from a marine macroalga Heterosiphonia japonica / [Li Sun, ShumeiWang, Xueqin Gong et al.] // Protein Expression and Purification. – 2009. – № 2. – P. 146–154.

Methods for Extraction, Isolation and Purification of C-phycocyanin: 50 years of Research in Review / [Verónica Cruz de Jesús, Gabriel Alfonso Gutiérrez-Rebolledo, Marcela Hernández-Ortega et al.] // International Journal of Food and Nutritional Science. – 2016. – № 3. – P. 1–10.

Full Text: PDF (EN)


  • There are currently no refbacks.